rpob genotyping result Search Results


escherichia coli castellani and chalmers  (ATCC)
99
ATCC escherichia coli castellani and chalmers
Escherichia Coli Castellani And Chalmers, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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genotyping  (Transnetyx)
99
Transnetyx genotyping
(A) Schematic of the murine interferon receptor ( Ifnr) gene locus and CRIPSR/Cas9 guide RNAs (gRNAs, red arrowheads) used for the deletion of a 192 kb genomic region of chromosome 16 (MMU16). Forward and reverse <t>genotyping</t> primers (FW and REV, black arrowheads) are indicated. Molecular location of genes based on GRCm38 reference genome is indicated on an ideogram of MMU16 cytogenetic regions colored according to Giemsa banding. (B) PCR of DNA from the founder (F0) mouse bearing the expected deletion (+) and negative controls [-; wildtype (WT) and Water (no DNA)]. (C) Representative Sanger sequencing of the single modified allele transmitted from the F0 male to the first generation of progeny (F1) after intercrossing with a WT female. (D) Whole genome sequencing followed by copy number variant analysis from the F0 mouse bearing a deletion of the Ifnr locus syntenic to human chromosome 21 (WT 1xIFNRs ) with the site of deletion on MMU16 denoted by arrow (left image) that is absent when two non-related C57BL/6N WT mice are compared (right image). *p<0.1 by CNV-seq. (E) Diagram of breeding strategy to reduce copy number of just four Ifnr genes (yellow line) triplicated in the Dp16 mouse model of Down syndrome by intercrossing Dp16 males with WT 1xIFNRs females. Littermate progeny can then be compared between Dp16, Dp16 normalized for just Ifnr dose (Dp16 2xIFNRs ), and WT controls.
Genotyping, supplied by Transnetyx, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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genotypic tests  (CH Instruments)
90
CH Instruments genotypic tests
(A) Schematic of the murine interferon receptor ( Ifnr) gene locus and CRIPSR/Cas9 guide RNAs (gRNAs, red arrowheads) used for the deletion of a 192 kb genomic region of chromosome 16 (MMU16). Forward and reverse <t>genotyping</t> primers (FW and REV, black arrowheads) are indicated. Molecular location of genes based on GRCm38 reference genome is indicated on an ideogram of MMU16 cytogenetic regions colored according to Giemsa banding. (B) PCR of DNA from the founder (F0) mouse bearing the expected deletion (+) and negative controls [-; wildtype (WT) and Water (no DNA)]. (C) Representative Sanger sequencing of the single modified allele transmitted from the F0 male to the first generation of progeny (F1) after intercrossing with a WT female. (D) Whole genome sequencing followed by copy number variant analysis from the F0 mouse bearing a deletion of the Ifnr locus syntenic to human chromosome 21 (WT 1xIFNRs ) with the site of deletion on MMU16 denoted by arrow (left image) that is absent when two non-related C57BL/6N WT mice are compared (right image). *p<0.1 by CNV-seq. (E) Diagram of breeding strategy to reduce copy number of just four Ifnr genes (yellow line) triplicated in the Dp16 mouse model of Down syndrome by intercrossing Dp16 males with WT 1xIFNRs females. Littermate progeny can then be compared between Dp16, Dp16 normalized for just Ifnr dose (Dp16 2xIFNRs ), and WT controls.
Genotypic Tests, supplied by CH Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bactec mgit 960  (Becton Dickinson)
90
Becton Dickinson bactec mgit 960
(A) Schematic of the murine interferon receptor ( Ifnr) gene locus and CRIPSR/Cas9 guide RNAs (gRNAs, red arrowheads) used for the deletion of a 192 kb genomic region of chromosome 16 (MMU16). Forward and reverse <t>genotyping</t> primers (FW and REV, black arrowheads) are indicated. Molecular location of genes based on GRCm38 reference genome is indicated on an ideogram of MMU16 cytogenetic regions colored according to Giemsa banding. (B) PCR of DNA from the founder (F0) mouse bearing the expected deletion (+) and negative controls [-; wildtype (WT) and Water (no DNA)]. (C) Representative Sanger sequencing of the single modified allele transmitted from the F0 male to the first generation of progeny (F1) after intercrossing with a WT female. (D) Whole genome sequencing followed by copy number variant analysis from the F0 mouse bearing a deletion of the Ifnr locus syntenic to human chromosome 21 (WT 1xIFNRs ) with the site of deletion on MMU16 denoted by arrow (left image) that is absent when two non-related C57BL/6N WT mice are compared (right image). *p<0.1 by CNV-seq. (E) Diagram of breeding strategy to reduce copy number of just four Ifnr genes (yellow line) triplicated in the Dp16 mouse model of Down syndrome by intercrossing Dp16 males with WT 1xIFNRs females. Littermate progeny can then be compared between Dp16, Dp16 normalized for just Ifnr dose (Dp16 2xIFNRs ), and WT controls.
Bactec Mgit 960, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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two-way anova  (SAS institute)
90
SAS institute two-way anova
(A) Schematic of the murine interferon receptor ( Ifnr) gene locus and CRIPSR/Cas9 guide RNAs (gRNAs, red arrowheads) used for the deletion of a 192 kb genomic region of chromosome 16 (MMU16). Forward and reverse <t>genotyping</t> primers (FW and REV, black arrowheads) are indicated. Molecular location of genes based on GRCm38 reference genome is indicated on an ideogram of MMU16 cytogenetic regions colored according to Giemsa banding. (B) PCR of DNA from the founder (F0) mouse bearing the expected deletion (+) and negative controls [-; wildtype (WT) and Water (no DNA)]. (C) Representative Sanger sequencing of the single modified allele transmitted from the F0 male to the first generation of progeny (F1) after intercrossing with a WT female. (D) Whole genome sequencing followed by copy number variant analysis from the F0 mouse bearing a deletion of the Ifnr locus syntenic to human chromosome 21 (WT 1xIFNRs ) with the site of deletion on MMU16 denoted by arrow (left image) that is absent when two non-related C57BL/6N WT mice are compared (right image). *p<0.1 by CNV-seq. (E) Diagram of breeding strategy to reduce copy number of just four Ifnr genes (yellow line) triplicated in the Dp16 mouse model of Down syndrome by intercrossing Dp16 males with WT 1xIFNRs females. Littermate progeny can then be compared between Dp16, Dp16 normalized for just Ifnr dose (Dp16 2xIFNRs ), and WT controls.
Two Way Anova, supplied by SAS institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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avaii  (New England Biolabs)
94
New England Biolabs avaii
(A) Schematic of the murine interferon receptor ( Ifnr) gene locus and CRIPSR/Cas9 guide RNAs (gRNAs, red arrowheads) used for the deletion of a 192 kb genomic region of chromosome 16 (MMU16). Forward and reverse <t>genotyping</t> primers (FW and REV, black arrowheads) are indicated. Molecular location of genes based on GRCm38 reference genome is indicated on an ideogram of MMU16 cytogenetic regions colored according to Giemsa banding. (B) PCR of DNA from the founder (F0) mouse bearing the expected deletion (+) and negative controls [-; wildtype (WT) and Water (no DNA)]. (C) Representative Sanger sequencing of the single modified allele transmitted from the F0 male to the first generation of progeny (F1) after intercrossing with a WT female. (D) Whole genome sequencing followed by copy number variant analysis from the F0 mouse bearing a deletion of the Ifnr locus syntenic to human chromosome 21 (WT 1xIFNRs ) with the site of deletion on MMU16 denoted by arrow (left image) that is absent when two non-related C57BL/6N WT mice are compared (right image). *p<0.1 by CNV-seq. (E) Diagram of breeding strategy to reduce copy number of just four Ifnr genes (yellow line) triplicated in the Dp16 mouse model of Down syndrome by intercrossing Dp16 males with WT 1xIFNRs females. Littermate progeny can then be compared between Dp16, Dp16 normalized for just Ifnr dose (Dp16 2xIFNRs ), and WT controls.
Avaii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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caption a7 strain genotype  (ATCC)
96
ATCC caption a7 strain genotype
(A) Schematic of the murine interferon receptor ( Ifnr) gene locus and CRIPSR/Cas9 guide RNAs (gRNAs, red arrowheads) used for the deletion of a 192 kb genomic region of chromosome 16 (MMU16). Forward and reverse <t>genotyping</t> primers (FW and REV, black arrowheads) are indicated. Molecular location of genes based on GRCm38 reference genome is indicated on an ideogram of MMU16 cytogenetic regions colored according to Giemsa banding. (B) PCR of DNA from the founder (F0) mouse bearing the expected deletion (+) and negative controls [-; wildtype (WT) and Water (no DNA)]. (C) Representative Sanger sequencing of the single modified allele transmitted from the F0 male to the first generation of progeny (F1) after intercrossing with a WT female. (D) Whole genome sequencing followed by copy number variant analysis from the F0 mouse bearing a deletion of the Ifnr locus syntenic to human chromosome 21 (WT 1xIFNRs ) with the site of deletion on MMU16 denoted by arrow (left image) that is absent when two non-related C57BL/6N WT mice are compared (right image). *p<0.1 by CNV-seq. (E) Diagram of breeding strategy to reduce copy number of just four Ifnr genes (yellow line) triplicated in the Dp16 mouse model of Down syndrome by intercrossing Dp16 males with WT 1xIFNRs females. Littermate progeny can then be compared between Dp16, Dp16 normalized for just Ifnr dose (Dp16 2xIFNRs ), and WT controls.
Caption A7 Strain Genotype, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mssrf10  (ATCC)
92
ATCC mssrf10
(A) Schematic of the murine interferon receptor ( Ifnr) gene locus and CRIPSR/Cas9 guide RNAs (gRNAs, red arrowheads) used for the deletion of a 192 kb genomic region of chromosome 16 (MMU16). Forward and reverse <t>genotyping</t> primers (FW and REV, black arrowheads) are indicated. Molecular location of genes based on GRCm38 reference genome is indicated on an ideogram of MMU16 cytogenetic regions colored according to Giemsa banding. (B) PCR of DNA from the founder (F0) mouse bearing the expected deletion (+) and negative controls [-; wildtype (WT) and Water (no DNA)]. (C) Representative Sanger sequencing of the single modified allele transmitted from the F0 male to the first generation of progeny (F1) after intercrossing with a WT female. (D) Whole genome sequencing followed by copy number variant analysis from the F0 mouse bearing a deletion of the Ifnr locus syntenic to human chromosome 21 (WT 1xIFNRs ) with the site of deletion on MMU16 denoted by arrow (left image) that is absent when two non-related C57BL/6N WT mice are compared (right image). *p<0.1 by CNV-seq. (E) Diagram of breeding strategy to reduce copy number of just four Ifnr genes (yellow line) triplicated in the Dp16 mouse model of Down syndrome by intercrossing Dp16 males with WT 1xIFNRs females. Littermate progeny can then be compared between Dp16, Dp16 normalized for just Ifnr dose (Dp16 2xIFNRs ), and WT controls.
Mssrf10, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mssrf38  (ATCC)
90
ATCC mssrf38
(A) Schematic of the murine interferon receptor ( Ifnr) gene locus and CRIPSR/Cas9 guide RNAs (gRNAs, red arrowheads) used for the deletion of a 192 kb genomic region of chromosome 16 (MMU16). Forward and reverse <t>genotyping</t> primers (FW and REV, black arrowheads) are indicated. Molecular location of genes based on GRCm38 reference genome is indicated on an ideogram of MMU16 cytogenetic regions colored according to Giemsa banding. (B) PCR of DNA from the founder (F0) mouse bearing the expected deletion (+) and negative controls [-; wildtype (WT) and Water (no DNA)]. (C) Representative Sanger sequencing of the single modified allele transmitted from the F0 male to the first generation of progeny (F1) after intercrossing with a WT female. (D) Whole genome sequencing followed by copy number variant analysis from the F0 mouse bearing a deletion of the Ifnr locus syntenic to human chromosome 21 (WT 1xIFNRs ) with the site of deletion on MMU16 denoted by arrow (left image) that is absent when two non-related C57BL/6N WT mice are compared (right image). *p<0.1 by CNV-seq. (E) Diagram of breeding strategy to reduce copy number of just four Ifnr genes (yellow line) triplicated in the Dp16 mouse model of Down syndrome by intercrossing Dp16 males with WT 1xIFNRs females. Littermate progeny can then be compared between Dp16, Dp16 normalized for just Ifnr dose (Dp16 2xIFNRs ), and WT controls.
Mssrf38, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mssrf7 a4 w 100 100 100  (ATCC)
91
ATCC mssrf7 a4 w 100 100 100
(A) Schematic of the murine interferon receptor ( Ifnr) gene locus and CRIPSR/Cas9 guide RNAs (gRNAs, red arrowheads) used for the deletion of a 192 kb genomic region of chromosome 16 (MMU16). Forward and reverse <t>genotyping</t> primers (FW and REV, black arrowheads) are indicated. Molecular location of genes based on GRCm38 reference genome is indicated on an ideogram of MMU16 cytogenetic regions colored according to Giemsa banding. (B) PCR of DNA from the founder (F0) mouse bearing the expected deletion (+) and negative controls [-; wildtype (WT) and Water (no DNA)]. (C) Representative Sanger sequencing of the single modified allele transmitted from the F0 male to the first generation of progeny (F1) after intercrossing with a WT female. (D) Whole genome sequencing followed by copy number variant analysis from the F0 mouse bearing a deletion of the Ifnr locus syntenic to human chromosome 21 (WT 1xIFNRs ) with the site of deletion on MMU16 denoted by arrow (left image) that is absent when two non-related C57BL/6N WT mice are compared (right image). *p<0.1 by CNV-seq. (E) Diagram of breeding strategy to reduce copy number of just four Ifnr genes (yellow line) triplicated in the Dp16 mouse model of Down syndrome by intercrossing Dp16 males with WT 1xIFNRs females. Littermate progeny can then be compared between Dp16, Dp16 normalized for just Ifnr dose (Dp16 2xIFNRs ), and WT controls.
Mssrf7 A4 W 100 100 100, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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armadillo-derived m. leprae  (Zensho Co Ltd)
90
Zensho Co Ltd armadillo-derived m. leprae
(A) Schematic of the murine interferon receptor ( Ifnr) gene locus and CRIPSR/Cas9 guide RNAs (gRNAs, red arrowheads) used for the deletion of a 192 kb genomic region of chromosome 16 (MMU16). Forward and reverse <t>genotyping</t> primers (FW and REV, black arrowheads) are indicated. Molecular location of genes based on GRCm38 reference genome is indicated on an ideogram of MMU16 cytogenetic regions colored according to Giemsa banding. (B) PCR of DNA from the founder (F0) mouse bearing the expected deletion (+) and negative controls [-; wildtype (WT) and Water (no DNA)]. (C) Representative Sanger sequencing of the single modified allele transmitted from the F0 male to the first generation of progeny (F1) after intercrossing with a WT female. (D) Whole genome sequencing followed by copy number variant analysis from the F0 mouse bearing a deletion of the Ifnr locus syntenic to human chromosome 21 (WT 1xIFNRs ) with the site of deletion on MMU16 denoted by arrow (left image) that is absent when two non-related C57BL/6N WT mice are compared (right image). *p<0.1 by CNV-seq. (E) Diagram of breeding strategy to reduce copy number of just four Ifnr genes (yellow line) triplicated in the Dp16 mouse model of Down syndrome by intercrossing Dp16 males with WT 1xIFNRs females. Littermate progeny can then be compared between Dp16, Dp16 normalized for just Ifnr dose (Dp16 2xIFNRs ), and WT controls.
Armadillo Derived M. Leprae, supplied by Zensho Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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truenat mtb (plus)  (MolBio Diagnostics)
90
MolBio Diagnostics truenat mtb (plus)
(A) Schematic of the murine interferon receptor ( Ifnr) gene locus and CRIPSR/Cas9 guide RNAs (gRNAs, red arrowheads) used for the deletion of a 192 kb genomic region of chromosome 16 (MMU16). Forward and reverse <t>genotyping</t> primers (FW and REV, black arrowheads) are indicated. Molecular location of genes based on GRCm38 reference genome is indicated on an ideogram of MMU16 cytogenetic regions colored according to Giemsa banding. (B) PCR of DNA from the founder (F0) mouse bearing the expected deletion (+) and negative controls [-; wildtype (WT) and Water (no DNA)]. (C) Representative Sanger sequencing of the single modified allele transmitted from the F0 male to the first generation of progeny (F1) after intercrossing with a WT female. (D) Whole genome sequencing followed by copy number variant analysis from the F0 mouse bearing a deletion of the Ifnr locus syntenic to human chromosome 21 (WT 1xIFNRs ) with the site of deletion on MMU16 denoted by arrow (left image) that is absent when two non-related C57BL/6N WT mice are compared (right image). *p<0.1 by CNV-seq. (E) Diagram of breeding strategy to reduce copy number of just four Ifnr genes (yellow line) triplicated in the Dp16 mouse model of Down syndrome by intercrossing Dp16 males with WT 1xIFNRs females. Littermate progeny can then be compared between Dp16, Dp16 normalized for just Ifnr dose (Dp16 2xIFNRs ), and WT controls.
Truenat Mtb (Plus), supplied by MolBio Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) Schematic of the murine interferon receptor ( Ifnr) gene locus and CRIPSR/Cas9 guide RNAs (gRNAs, red arrowheads) used for the deletion of a 192 kb genomic region of chromosome 16 (MMU16). Forward and reverse genotyping primers (FW and REV, black arrowheads) are indicated. Molecular location of genes based on GRCm38 reference genome is indicated on an ideogram of MMU16 cytogenetic regions colored according to Giemsa banding. (B) PCR of DNA from the founder (F0) mouse bearing the expected deletion (+) and negative controls [-; wildtype (WT) and Water (no DNA)]. (C) Representative Sanger sequencing of the single modified allele transmitted from the F0 male to the first generation of progeny (F1) after intercrossing with a WT female. (D) Whole genome sequencing followed by copy number variant analysis from the F0 mouse bearing a deletion of the Ifnr locus syntenic to human chromosome 21 (WT 1xIFNRs ) with the site of deletion on MMU16 denoted by arrow (left image) that is absent when two non-related C57BL/6N WT mice are compared (right image). *p<0.1 by CNV-seq. (E) Diagram of breeding strategy to reduce copy number of just four Ifnr genes (yellow line) triplicated in the Dp16 mouse model of Down syndrome by intercrossing Dp16 males with WT 1xIFNRs females. Littermate progeny can then be compared between Dp16, Dp16 normalized for just Ifnr dose (Dp16 2xIFNRs ), and WT controls.

Journal: bioRxiv

Article Title: Interferon receptor gene dosage determines diverse hallmarks of Down syndrome

doi: 10.1101/2022.02.03.478982

Figure Lengend Snippet: (A) Schematic of the murine interferon receptor ( Ifnr) gene locus and CRIPSR/Cas9 guide RNAs (gRNAs, red arrowheads) used for the deletion of a 192 kb genomic region of chromosome 16 (MMU16). Forward and reverse genotyping primers (FW and REV, black arrowheads) are indicated. Molecular location of genes based on GRCm38 reference genome is indicated on an ideogram of MMU16 cytogenetic regions colored according to Giemsa banding. (B) PCR of DNA from the founder (F0) mouse bearing the expected deletion (+) and negative controls [-; wildtype (WT) and Water (no DNA)]. (C) Representative Sanger sequencing of the single modified allele transmitted from the F0 male to the first generation of progeny (F1) after intercrossing with a WT female. (D) Whole genome sequencing followed by copy number variant analysis from the F0 mouse bearing a deletion of the Ifnr locus syntenic to human chromosome 21 (WT 1xIFNRs ) with the site of deletion on MMU16 denoted by arrow (left image) that is absent when two non-related C57BL/6N WT mice are compared (right image). *p<0.1 by CNV-seq. (E) Diagram of breeding strategy to reduce copy number of just four Ifnr genes (yellow line) triplicated in the Dp16 mouse model of Down syndrome by intercrossing Dp16 males with WT 1xIFNRs females. Littermate progeny can then be compared between Dp16, Dp16 normalized for just Ifnr dose (Dp16 2xIFNRs ), and WT controls.

Article Snippet: Briefly, genomic DNA was prepared from 1-2 mm of toe, tail or ear tissue using the HotSHOT method ( ) then run through PCR according to Data S4, Tabs B-C or outsourced for automated genotyping by RT-PCR with specific probes designed for each gene (Transnetyx, Cordova, TN).

Techniques: Sequencing, Modification, Variant Assay